Journal: Cells
Article Title: Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells
doi: 10.3390/cells11091379
Figure Lengend Snippet: The CD38+ cell population expresses hepatic stellate cell specific intracellular markers GFAP and Nestin. CD38 expression of backgated GFAP+ Nestin+ and violet-induced (AF)+ populations after isotype control (upper panel) and antibody (lower panel) directed against CD38. It appeared that the majority of CD38+ cells were positive for Nestin and GFAP and autofluorescent. Proportions (of viable cells) are expressed as a mean ± SD, n = 2.
Article Snippet: Phycoerythrin-cyanine7-conjugated anti-mouse CD38, phycoerythrin-conjugated anti-mouse Nestin, Alexa Fluor 488-conjugated anti-mouse glial fibrillary acidic protein (GFAP), phycoerythrin-cyanine7-conjugated rat IgG2aκ isotype control, phycoerythrin-conjugated mouse IgG2a isotype control, and DRAQ7 were obtained from Thermo Fisher Scientific.
Techniques: Expressing, Control